The valuations of properties and the negotiations with property owners are two major tasks in the right-of-way acquisition process for transportation projects. If improved, those tasks can increase the overall project delivery efficiency. The Texas Department of Transportation (TxDOT) funded a research project that aimed to recommend some best practices for successful valuations and negotiations in Texas. The authors reviewed the different strategies and procedures followed in TxDOT by conducting interviews and surveys with right-of-way personnel. Guidelines supported by recommended practices were identified for both valuation and negotiation and then reviewed and validated by experts. Lastly, implementation guides were developed.